The ministry at a press conference said that it would take three months of surveillance in the affected areas to satisfy the World Organization for Animal Health (WOAH) standards.
Currently the areas that have been properly disinfected by the culling teams employed by the ministry have been issued sanitary certificates.
"We will be in constant surveillance of the areas where we have issued sanitary certificates for a period of three months. Only then can we be absolutely sure," said Dr Prabhakar Pathak.
Pathak further said that the disease was not found in any human.
Bird flu was first spotted on January 16 at Kakarbhitta ward no 10 of Mechinagar Municipality in Jhapa district.
"Other than the initial samples from Mechinagar Municipality, the other diseased birds from around Nepal were found to be infected with a virulent version of Ranikhet disease and not bird flu," expressed Pathak.
Detecting bird flu